ABSTRACT
OBJECTIVE@#To determine whether topical applications of thiosulfinate-enriched Allium sativum extract (TASE) can accelerate acute cutaneous wound healing (WH) in a murine model.@*METHODS@#Keratinocyte viability and in vitro wound closure were assessed in keratinocyte cultures. Effects of topical TASE (0.5 μg/mL of allicin in 97% ethanol) on acute cutaneous WH were determined in a murine model of acute cutaneous wound. Twelve mice were alternately assigned to the vehicle- and TASE-treated groups (n=6 per group). Expression levels of mRNA for keratinocyte differentiation marker-related proteins (filaggrin, loricrin and involucrin) and lipid synthetic enzymes (elongation of very long chain fatty acids protein 4 (ELOVL4), fatty acid synthase (FA2H), 3-hydroxy- 3-methyl-glutaryl-coenzyme A reductase (HMGCoA), and serine palmitoyltransferase (SPT)) were assessed using real-time quantitative polymerase chain reaction on day 3 and 8 after wounding, while transepidermal water loss (TEWL) rates were measured in wounded areas.@*RESULTS@#TASE accelerated WH both in vivo (40% vs. 22% reduction in wound area, P<0.01) and in vitro (90% vs. 65% reduction in wound area, P<0.01). Moreover, topical applications of TASE upregulated the expression levels of epidermal mRNA for ELOVL4, HMGCoA, SPT, filaggrin, loricrin and involucrin (P<0.05 vs. vehicle-treated controls) on day 3 after wounding. Likewise, TASE significantly lowered TEWL rates in comparison with vehicle alone on day 8 (33.06±2.09 g/(m@*CONCLUSIONS@#Topical applications of TASE stimulated keratinocyte proliferation and formation of epidermal permeability barrier function, leading to acceleration of acute cutaneous WH. Topical products containing TASE could be used to manage acute cutaneous WH.
ABSTRACT
Atopic dermatitis (AD) is among the most common skin disorders in humans. Although a variety of regimens are available for the treatment of AD, preventive approaches are limited. Recent studies have demonstrated that certain naturally-occurring herbal medicines are effective in preventing the development of AD via divergent mechanisms, such as inhibiting cytokine and chemokine expression, IgE production, inflammatory cell infiltration, histamine release, and/or enhancement of epidermal permeability barrier function. Yet, they exhibit few adverse effects. Since herbal medicines are widely available, inexpensive and generally safe, they could represent an ideal approach for preventing the development of AD, in both highly developed and developing countries.
Subject(s)
Animals , Humans , Chemokines , Metabolism , Dermatitis, Atopic , Disease Models, Animal , Herbal Medicine , Immunoglobulin E , Metabolism , Inflammation , PathologyABSTRACT
OBJECTIVE: To explore the current status and influencing factors of regulatory emotional self-efficacy(RESE) among graduate students of medical college. METHODS: A cluster sampling method was used to select 3 225 postgraduates in class of 2015 and 2016 from a medical college as research subjects. A RESE survey was conducted using the Regulatory Emotional Self-Efficacy Scale. RESULTS: The score of RESE was 66.8±7.6. The score of perceived self-efficiency in expressing emotion was 25.7±3.0. The score of perceived self-efficiency in managing negative emotion was 41.1±6.0. Multivariate linear regression analysis showed that the RESE scores of the first grade graduates were higher than those of the second-grade. The RESE scores of students whose mothers′ educational level above primary school were higher than those with their mothers′ educational level being illiteracy and below primary school. The RESE scores of those being more optimistic about job prospects were higher than those being less optimistic about job prospects. The RESE scores of those with large amount of exercise were higher than those with small amount of exercise. The partial regression coefficients were-0.65, 1.03, 4.33 and 1.87(P<0.05). CONCLUSION: The level of RESE in medical college graduate students is high. The grades of medical graduates, attitudes toward employment prospects, physical exercise and mother′s educational level are the influencing factors of their RESE.
ABSTRACT
Although a variety of regimens are available for the treatment of atopic dermatitis (AD), severe adverse reactions and unpopular costs often limit their usage. In contrast, certain inexpensive, naturally-occurring ingredients are proven effective for AD with fewer side effects. The beneficial effects of these ingredients can be attributed to inhibition of cytokine and chemokine expression, IgE production, inflammatory cell infiltration, histamine release, and/or the enhancement of epidermal permeability barrier function. Since herbal medicines are widely available, inexpensive and generally safe, they could be valuable alternatives for the treatment of AD, particularly for those patients who are not suitable for the utilization of immune modulators. In this review, we summarize the therapeutic benefits of natural ingredients for the treatment of AD and the mechanisms of their actions.